Osaka Kyoiku University Researcher Information
日本語 | English
研究者業績
基本情報
- 所属
- 大阪教育大学 理事・副学長
- 学位
- 修士(農学)(東北大学)Ph. Doctor(Agriculture)(Tohoku University)博士(農学)(東北大学)
- 研究者番号
- 10314444
- J-GLOBAL ID
- 200901081252736172
- researchmap会員ID
- 1000248485
- 外部リンク
経歴
8-
2020年4月 - 2024年3月
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2022年4月
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2008年4月 - 2016年3月
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2014年4月
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2007年4月 - 2014年3月
学歴
2-
1996年4月 - 1999年3月
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- 1994年
委員歴
1-
2007年
受賞
3-
2005年8月
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2001年
論文
91-
Scientific Reports 14(1) 9656 2024年4月26日 査読有りAbstract Weedy rice is a major problem in paddy fields around the world. It is well known that weedy rice appears to grow faster and mature earlier than cultivated rice. It is possible that differences in the root microbial genetics are correlated with this characteristic. This study incorporated 16S rRNA amplicon sequencing to study the microbial composition in the rhizosphere and endosphere of rice root. No significant difference was found between the microbiota associated with weedy and cultivated rice lines grown in the same field. It was found that the endosphere had less microbial diversity compared to the rhizosphere. The major groups of bacteria found in the endosphere are from the phylum Proteobacteria, Myxococcota, Chloroflexota, and Actinobacteria. In addition, by analyzing the microbiome of japonica rice grown in the field in a temperate climate, we found that despite differences in genotype and location, some bacterial taxa were found to be common and these members of the putative rice core microbiome can also be detected by in situ hybridization. The delineation of a core microbiome in the endosphere of rice suggests that these bacterial taxa might be important in the life cycle of a wide range of rice types.
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Plant Reproduction 2023年4月26日Abstract Key message S29 haplotype does not require the MLPK function for self-incompatibility in Brassica rapa. Abstract Self-incompatibility (SI) in Brassicaceae is regulated by the self-recognition mechanism, which is based on the S-haplotype-specific direct interaction of the pollen-derived ligand, SP11/SCR, and the stigma-side receptor, SRK. M locus protein kinase (MLPK) is known to be one of the positive effectors of the SI response. MLPK directly interacts with SRK, and is phosphorylated by SRK in Brassica rapa. In Brassicaceae, MLPK was demonstrated to be essential for SI in B. rapa and Brassica napus, whereas it is not essential for SI in Arabidopsis thaliana (with introduced SRK and SP11/SCR from related SI species). Little is known about what determines the need for MLPK in SI of Brassicaceae. In this study, we investigated the relationship between S-haplotype diversity and MLPK function by analyzing the SI phenotypes of different S haplotypes in a mlpk/mlpk mutant background. The results have clarified that in B. rapa, all the S haplotypes except the S29 we tested need the MLPK function, but the S29 haplotype does not require MLPK for the SI. Comparative analysis of MLPK-dependent and MLPK-independent S haplotype might provide new insight into the evolution of S-haplotype diversity and the molecular mechanism of SI in Brassicaceae.
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Plants (Basel) 10 2467 2021年11月15日 査読有り
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Genes and Genetic Systems 96 129-139 2021年6月18日 査読有り
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Nature Communications 11(1) 4916 2020年10月1日 査読有り
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Frontiers in Plant Science 11 576140-576140 2020年9月11日 査読有り
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Genes and Genetic Systems 95 Article ID: 19-00050 2020年6月4日 査読有り
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Genes and Genetic Systems 94(94) 167-176 2019年8月31日 査読有り
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Entomological Science 22 167-172 2019年 査読有り
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Journal of Insect Biotechnology and Sericology 87 61-69 2018年 査読有り
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Genes and Genetic Systems 93(5) 209-220 2018年 査読有り
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NATURE PLANTS 3(7) 17096 2017年7月 査読有り
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Nature Plants 3 16206 2016年12月22日 査読有り
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GENES & GENETIC SYSTEMS 91(2) 111-125 2016年4月 査読有り招待有り
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GENES & GENETIC SYSTEMS 91(2) 97-109 2016年4月 査読有り招待有り
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Plant & cell physiology 56(4) 663-73 2015年4月 査読有りPollination is an important early step in sexual plant reproduction. In Arabidopsis thaliana, sequential pollination events, from pollen adhesion onto the stigma surface to pollen tube germination and elongation, occur on the stigmatic papilla cells. Following successful completion of these events, the pollen tube penetrates the stigma and finally fertilizes a female gametophyte. The pollination events are thought to be initiated and regulated by interactions between papilla cells and pollen. Here, we report the characterization of gene expression profiles of unpollinated (UP), compatible pollinated (CP) and incompatible pollinated (IP) papilla cells in A. thaliana. Based on cell type-specific transcriptome analysis from a combination of laser microdissection and RNA sequencing, 15,475, 17,360 and 16,918 genes were identified as expressed in UP, CP and IP papilla cells, respectively, and, of these, 14,392 genes were present in all three data sets. Differentially expressed gene (DEG) analyses identified 147 and 71 genes up-regulated in CP and IP papilla cells, respectively, and 115 and 46 genes down-regulated. Gene Ontology and metabolic pathway analyses revealed that papilla cells play an active role as the female reproductive component in pollination, particularly in information exchange, signal transduction, internal physiological changes and external morphological modification. This study provides fundamental information on the molecular mechanisms involved in pollination in papilla cells, furthering our understanding of the reproductive role of papilla cells.
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PLANT AND CELL PHYSIOLOGY 56(1) e9 2015年1月 査読有り
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The Nucleus 58 207-210 2015年 査読有り
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NEW PHYTOLOGIST 201(3) 973-981 2014年2月 査読有り
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PLANT BIOTECHNOLOGY 31(1) 67-70 2014年 査読有り
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Plant & cell physiology 54(11) 1894-906 2013年11月 査読有りPollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.
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GENES & GENETIC SYSTEMS 88(5) 279-287 2013年10月 査読有り
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Annals of Botany 112(1) 115-122 2013年7月 査読有り
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GENE 514(2) 75-81 2013年2月 査読有り
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G3: Genes, Genomes, Genetics 3(4) 719-726 2013年 査読有り
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G3-GENES GENOMES GENETICS 2(6) 643-651 2012年6月 査読有り
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BREEDING SCIENCE 62(2) 170-177 2012年6月 査読有り
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PLANT CELL REPORTS 31(4) 621-628 2012年4月 査読有り
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Proceeding of Jpnanese Acaddemy of Science, Ser. B 88(10) 519-535 2012年 査読有りSelf-incompatibility (SI) is defined as the inability to produce zygotes after self-pollination in a fertile hermaphrodite plant, which has stamens and pistils in the same flower. This structural organization of the hermaphrodite flower increases the risk of self-pollination, leading to low genetic diversity. To avoid this problem plants have established several pollination systems, among which the most elegant system is surely SI. The SI trait can be observed in Brassica crops, including cabbage, broccoli, turnip and radish. To produce hybrid seed of these crops efficiently, the SI trait has been employed in an agricultural context. From another point of view, the recognition reaction of SI during pollen-stigma interaction is an excellent model system for cell-cell communication and signal transduction in higher plants. In this review, we describe the molecular mechanisms of SI in Brassicaceae, which have been dissected by genetic, physiological, and biological approaches, and we discuss the future prospects in relation to associated scientific fields and new technologies.<BR><BR>(Communicated by Tsuneyoshi KUROIWA, M.J.A.)
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PLANT CELL REPORTS 30(12) 2293-2301 2011年12月 査読有り
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PLANT PHYSIOLOGY 157(3) 1327-1341 2011年11月 査読有り
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CHROMOSOME RESEARCH 19(5) 591-605 2011年7月 査読有り
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PLANT JOURNAL 66(5) 890-902 2011年6月 査読有り
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PloS one 6(10) e26162 2011年 査読有りCo-expression networks systematically constructed from large-scale transcriptome data reflect the interactions and functions of genes with similar expression patterns and are a powerful tool for the comprehensive understanding of biological events and mining of novel genes. In Arabidopsis (a model dicot plant), high-resolution co-expression networks have been constructed from very large microarray datasets and these are publicly available as online information resources. However, the available transcriptome data of rice (a model monocot plant) have been limited so far, making it difficult for rice researchers to achieve reliable co-expression analysis. In this study, we performed co-expression network analysis by using combined 44 K agilent microarray datasets of rice, which consisted of 33 laser microdissection (LM)-microarray datasets of anthers, and 143 spatiotemporal transcriptome datasets deposited in RicexPro. The entire data of the rice co-expression network, which was generated from the 176 microarray datasets by the Pearson correlation coefficient (PCC) method with the mutual rank (MR)-based cut-off, contained 24,258 genes and 60,441 genes pairs. Using these datasets, we constructed high-resolution co-expression subnetworks of two specific biological events in the anther, "meiosis" and "pollen wall synthesis". The meiosis network contained many known or putative meiotic genes, including genes related to meiosis initiation and recombination. In the pollen wall synthesis network, several candidate genes involved in the sporopollenin biosynthesis pathway were efficiently identified. Hence, these two subnetworks are important demonstrations of the efficiency of co-expression network analysis in rice. Our co-expression analysis included the separated transcriptomes of pollen and tapetum cells in the anther, which are able to provide precise information on transcriptional regulation during male gametophyte development in rice. The co-expression network data presented here is a useful resource for rice researchers to elucidate important and complex biological events.
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GENES & GENETIC SYSTEMS 85(6) 377-382 2010年12月 査読有り
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Nature 466(7309) 983-986 2010年8月 査読有り
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PLANT AND CELL PHYSIOLOGY 51(6) 981-996 2010年6月 査読有り
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NATURE 464(7293) 1342-1346 2010年4月 査読有り
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GENES & GENETIC SYSTEMS 85(2) 87-96 2010年4月 査読有り
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GENES & GENETIC SYSTEMS 85(2) 107-120 2010年4月 査読有り
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JOURNAL OF CEREAL SCIENCE 51(2) 182-188 2010年3月 査読有り
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PLANT BIOTECHNOLOGY 26(4) 421-425 2009年9月 査読有り
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PLANT CELL REPORTS 28(5) 759-768 2009年5月 査読有り
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MOLECULAR GENETICS AND GENOMICS 280(4) 287-292 2008年10月 査読有り
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Plant & cell physiology 49(10) 1407-16 2008年10月 査読有りIn flowering plants, the male gametophyte, the pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. Expression profiles of 11 known tapetum specific-genes were consistent with previous reports. Based on their spatial and temporal expression patterns, 140 genes which had been previously defined as anther specific were further classified as male gametophyte specific (71 genes, 51%), tapetum-specific (seven genes, 5%) or expressed in both male gametophyte and tapetum (62 genes, 44%). These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum.
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Plant & cell physiology 49(10) 1417-28 2008年10月 査読有りThe male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation.
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GENES & GENETIC SYSTEMS 83(3) 281-284 2008年6月 査読有り
MISC
31書籍等出版物
6-
Springer-Verlag 2008年 (ISBN: 9783540684862)
共同研究・競争的資金等の研究課題
25-
日本学術振興会 科学研究費助成事業 2020年4月 - 2023年3月
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日本学術振興会 科学研究費助成事業 2016年6月 - 2021年3月
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日本学術振興会 科学研究費助成事業 2014年4月 - 2017年3月
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日本学術振興会 科学研究費助成事業 2013年4月 - 2017年3月
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2013年 - 2017年