Osaka Kyoiku University Researcher Information
日本語 | English
Curriculum Vitaes
Profile Information
- Affiliation
- Professor, Division of Math, Sciences, and Information Technology in Education, Osaka Kyoiku University
- Degree
- 修士(農学)(東北大学)Ph. Doctor(Agriculture)(Tohoku University)博士(農学)(東北大学)
- Researcher number
- 10314444
- J-GLOBAL ID
- 200901081252736172
- researchmap Member ID
- 1000248485
- External link
Research Areas
2Research History
7-
Apr, 2020
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Apr, 2008 - Mar, 2016
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Apr, 2014
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Apr, 2007 - Mar, 2014
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Apr, 2012
Education
2-
Apr, 1996 - Mar, 1999
Committee Memberships
1-
2007
Awards
3-
Aug, 2005
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2001
Papers
92-
Plant Reproduction, Apr 26, 2023Abstract Key message S29 haplotype does not require the MLPK function for self-incompatibility in Brassica rapa. Abstract Self-incompatibility (SI) in Brassicaceae is regulated by the self-recognition mechanism, which is based on the S-haplotype-specific direct interaction of the pollen-derived ligand, SP11/SCR, and the stigma-side receptor, SRK. M locus protein kinase (MLPK) is known to be one of the positive effectors of the SI response. MLPK directly interacts with SRK, and is phosphorylated by SRK in Brassica rapa. In Brassicaceae, MLPK was demonstrated to be essential for SI in B. rapa and Brassica napus, whereas it is not essential for SI in Arabidopsis thaliana (with introduced SRK and SP11/SCR from related SI species). Little is known about what determines the need for MLPK in SI of Brassicaceae. In this study, we investigated the relationship between S-haplotype diversity and MLPK function by analyzing the SI phenotypes of different S haplotypes in a mlpk/mlpk mutant background. The results have clarified that in B. rapa, all the S haplotypes except the S29 we tested need the MLPK function, but the S29 haplotype does not require MLPK for the SI. Comparative analysis of MLPK-dependent and MLPK-independent S haplotype might provide new insight into the evolution of S-haplotype diversity and the molecular mechanism of SI in Brassicaceae.
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Plants (Basel), 10 2467, Nov 15, 2021 Peer-reviewed
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Genes and Genetic Systems, 96 129-139, Jun 18, 2021 Peer-reviewed
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Nature Communications, 11(1) 4916, Oct 1, 2020 Peer-reviewed<title>Abstract</title> Self-incompatibility (SI) is a breeding system that promotes cross-fertilization. In <italic>Brassica</italic>, pollen rejection is induced by a haplotype-specific interaction between pistil determinant SRK (<italic>S</italic> receptor kinase) and pollen determinant SP11 (<italic>S</italic>-locus Protein 11, also named SCR) from the <italic>S</italic>-locus. Although the structure of the <italic>B. rapa S</italic>9-SRK ectodomain (eSRK) and <italic>S</italic>9-SP11 complex has been determined, it remains unclear how SRK discriminates self- and nonself-SP11. Here, we uncover the detailed mechanism of self/nonself-discrimination in <italic>Brassica</italic> SI by determining the <italic>S</italic>8-eSRK–<italic>S</italic>8-SP11 crystal structure and performing molecular dynamics (MD) simulations. Comprehensive binding analysis of eSRK and SP11 structures reveals that the binding free energies are most stable for cognate eSRK–SP11 combinations. Residue-based contribution analysis suggests that the modes of eSRK–SP11 interactions differ between intra- and inter-subgroup (a group of phylogenetically neighboring haplotypes) combinations. Our data establish a model of self/nonself-discrimination in <italic>Brassica</italic> SI.
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Frontiers in Plant Science, 11 576140-576140, Sep 11, 2020 Peer-reviewedSelf-compatibility in Arabidopsis thaliana represents the relatively recent disruption of ancestral obligate cross pollination, recognized as one of the prevalent evolutionary pathways in flowering plants, as noted by Darwin. Our previous study found that inversion of the male specificity gene (SP11/SCR) disrupted self-incompatibility, which was restored by overexpressing the SCR with the reversed inversion. However, SCR in A. thaliana has other mutations aside from the pivotal inversion, in both promoter and coding regions, with probable effects on transcriptional regulation. To examine the functional consequences of these mutations, we conducted reciprocal introductions of native promoters and downstream sequences from orthologous loci of self-compatible A. thaliana and self-incompatible A. halleri. Use of this inter-species pair enabled us to expand the scope of the analysis to transcriptional regulation and deletion in the intron, in addition to inversion in the native genomic background. Initial analysis revealed that A. thaliana has a significantly lower basal expression level of SCR transcripts in the critical reproductive stage compared to that of A. halleri, suggesting that the promoter was attenuated in inducing transcription in A. thaliana. However, in reciprocal transgenic experiments, this A. thaliana promoter was able to restore partial function if coupled with the functional A. halleri coding sequence, despite extensive alterations due to the self-compatible mode of reproduction in A. thaliana. This represents a synergistic effect of the promoter and the inversion resulting in fixation of self-compatibility, primarily enforced by disruption of SCR. Our findings elucidate the functional and evolutionary context of the historical transition in A. thaliana thus contributing to the understanding of the molecular events leading to development of self-compatibility.
Misc.
31-
Memoirs of Osaka Kyoiku University. Educational Science, 71 463-472, Feb 28, 2023 Lead author
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日本植物生理学会年会要旨集, 52nd 170-0236, Mar 11, 2011Many flowering plants have evolved self-incompatibility (SI) systems to prevent inbreeding. SI in Brassica is controlled by a large number of haplotypes at the S-locus. Each S-haplotype encodes the pollen-borne ligand S-locus protein 11 (SP11, also named S-locus cystein rich protein, SCR) and its stigmatic receptor S-receptor protein kinase (SRK). The SI phenotype of pollen is determined by the dominance relationships between the two S-haplotypes the plant carries. Based on these relationships, the S-haplotypes in Brassica have been classified into two groups; the pollen-dominant (class I) and the pollen-recessive (class II) S-haplotypes. Pollen-dominant S-haplotypes are generally codominant with each other, and they are always dominant over recessive S-haplotypes in S-heterozygotes. Previously we have shown that the expression of recessive SP11 allele is silenced as a result of tapetum-specific DNA methylation in its promoter region in the dominant/recessive S-heterozygotes. Here we show that this methylation is controlled by trans-acting small RNA encoded in the flanking region of dominant SP11 allele.
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GENES & GENETIC SYSTEMS, 85(6) 423-423, Dec, 2010
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GENES & GENETIC SYSTEMS, 85(5) 297-310, Oct, 2010 Peer-reviewedIn the last decade, a variety of innovations of emerging technologies in science have been accomplished. Advanced research environment in plant science has made it possible to obtain whole genome sequence in plant species. But now we recognize this by itself is not sufficient to understand the overall biological significance. Since Gregor Mendel established a principle of genetics, known as Mendel's Laws of Inheritance, genetics plays a prominent role in life science, and this aspect is indispensable even in modern plant biology. In this review, we focus on achievements of genetics on plant sexual reproduction research in the last decade and discuss the role of genetics for the coming decade. It is our hope that this will shed light on the importance of genetics in plant biology and provide valuable information to plant biologists.
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PLANT AND CELL PHYSIOLOGY, 50(11) 1857-1864, Nov, 2009 Peer-reviewedSexual reproduction is an important biological event not only for evolution but also for breeding in plants. It is a well known fact that Charles Darwin (18091882) was interested in the reproduction system of plants as part of his concept of species and evolution. His keen observation and speculation is timeless even in the current post-genome era. In the Darwin anniversary year of 2009, I have summarized recent molecular genetic studies of plant reproduction, focusing especially on male gametophyte development, pollination and fertilization. We are just beginning to understand the molecular mechanisms of the elaborate reproduction system in flowering plants, which have been a mystery for 100 years.
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GENES & GENETIC SYSTEMS, 81(6) 458-458, Dec, 2006
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Floriculture Ornamental and Plant Biotechnology, 1 552-555, 2006
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育種学研究, 2(2) 245, Sep, 2000
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Plant Biotechnology, 16(4) 263-272, 1999 Peer-reviewedMany angiosperm plants express self- incompatibility (SI), through which they can recognize selfpollen and restrict fertilization to non-self-pollen. In species of Brassica, SI is sporophytically expressed, regulated by a single locus, S, with multiple alleles. Two stigma- specific genes, SLG and SRK, both of which locate at the S locus, are believed to play a role in the recognition reaction on the stigma side. Reviewed here are findings about SLG and SRK genes, the molecular characterization of Smultigene family, the genomic structure of S locus, and some aspects on signal transfer by the proteins encoded by these genes.
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PLANT PHYSIOLOGY, 114(3) 1232-1232, Jul, 1997
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Recent Research Developments in Agricultural and Biological Chemistry., 1 235-242, 1997 Peer-reviewed
Books and Other Publications
6-
Springer-Verlag, 2008 (ISBN: 9783540684862)
Research Projects
25-
Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2023
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Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, Jun, 2016 - Mar, 2021
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Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2017
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Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, Apr, 2013 - Mar, 2017
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2013 - 2017